Oligosaccharides

 

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Dihydrobenzoin
Oligosaccharides

 


Laser Polarimetry, Refractive Index and UV Absorbance

Here we present a comparison of separations of a mixture of malto-oligosaccharides with DP 4-10. In figure below we can see that the response of the polarimetric detector offers an excellent response for this oligosaccharide series. However, with the refractive index detector and the UV detector the only measurable response is for the solvent front.

                           

      Laser Polarimeter                 Refractive Index                          UV Absorbance

The separation was performed on a Cyclobond I 2000 column with a mobile phase of acetonitrile/water = 70/30, 1 ml/min.